DNA Methylation - Thyroid & Fluoride
Posted: Thu Jun 23, 2022 6:57 pm
DNA Methylation & Thyroid Status
Just a short list of relevant papers:
Bunker SK, Dandapat J, Chainy GBN, Sahoo SK, Nayak PK - "Neonatal Exposure to 6-n-Propyl-Thiouracil, an Anti-Thyroid Drug, Alters Expression of Hepatic DNA Methyltransferases, Methyl CpG-Binding Proteins, Gadd45a, p53, and PCNA in Adult Male Rats" Eur Thyroid J 6(6):281-291 (2017)
doi: 10.1159/000479681
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704726/
"The results suggest that neonatal exposure of male rats to PTU resulted in alteration in the expression of proteins that are associated with DNA methylation and genome stabilization in adult rat liver."
Lafontaine N, Campbell PJ, Castillo-Fernandez JE, Mullin S, Lim EM, Kendrew P, Lewer M, Brown SJ, Huang RC, Melton PE, Mori TA, Beilin LJ, Dudbridge F, Spector TD, Wright MJ, Martin NG, McRae AF, Panicker V, Zhu G, Walsh JP, Bell JT, Wilson SG - "Epigenome-Wide Association Study of Thyroid Function Traits Identifies Novel Associations of fT3 With KLF9 and DOT1L" J Clin Endocrinol Metab 106(5):e2191-e2202 (2021) doi: 10.1210/clinem/dgaa975
https://academic.oup.com/jcem/article/1 ... 91/6114572
"We identified 23 differentially methylated regions associated with fT3, fT4, or TSH...This study has demonstrated associations between blood-based DNAm and both fT3 and TSH."
Qu M, Wan S, Wu H, Ren B, Chen Y, Liu L, Shen H - "The Whole Blood DNA Methylation Patterns of Extrinsic Apoptotic Signaling Pathway Related Genes in Autoimmune Thyroiditis among Areas with Different Iodine Levels" Br J Nutr 9:1-35 (2022). doi: 10.1017/S0007114522000721
https://www.cambridge.org/core/journals ... 53D0CB4D56
Wan S, Liu L, Ren B, Qu M, Wu H, Jiang W, Wang X, Shen H - "DNA Methylation Patterns in the HLA-DPB1 and PDCD1LG2 Gene Regions in Patients with Autoimmune Thyroiditis from Different Water Iodine Areas" Thyroid 31(11):1741-1748 (2021)
https://www.liebertpub.com/doi/10.1089/thy.2021.0221
" There were multiple differential methylation sites in the HLA-DPB1 and PDCD1LG2 genes between the case and control population with different water iodine levels."
https://www.tandfonline.com/doi/abs/10. ... ode=iaut20
"Multiple regression analysis indicated that patients with HD, including severe or mild HD, showed higher methylation levels at the -426 CpG site than control subjects. Patients with intractable GD showed lower methylation levels at the -664 and -666 CpG sites than patients with GD in remission. In conclusion, IL6 gene methylation levels were related to the susceptibility to HD and the intractability of GD."
Morita E, Watanabe M, Inoue N, Hashimoto H, Haga E, Hidaka Y, Iwatani Y - "Methylation levels of the TNFA gene are different between Graves' and Hashimoto's diseases and influenced by the TNFA polymorphism" Autoimmunity 51(3):118-125 (2018)
https://www.tandfonline.com/doi/abs/10. ... 18.1448078
Luo J, Wang X, Yuan L, Guo L - "Genome‑wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism" Clin Epigenetics 13(1):123 (2021) doi: 10.1186/s13148-021-01109-2
https://clinicalepigeneticsjournal.biom ... 21-01109-2
Fluoride
Short list:
Chen N, Zhang J, Yin C, Liao Y, Song L, Hu T, Pan X - "Abnormal methylation of Mill1 gene regulates osteogenic differentiation involved in various phenotypes of skeletal fluorosis in rats and methionine intervention" Ecotoxicol Environ Saf 290:117519 (2024) doi: 10.1016/j.ecoenv.2024.117519.
https://www.sciencedirect.com/science/a ... 1324015951
"The results indicated that under normal nutrition and fluoride exposure followed by methionine intervention, the methylation levels of the Mill1 gene increased, whereas its high expression and enhanced osteogenic differentiation were restrained."
Chen L, Yin N, Ding Y, Zhang ML, Li M, Zhong JJ, Feng SM - "Effects of fluoride on the proliferation and activation of osteoblasts by regulating methylation of the DNA repair genes MGMT and MLH1" Regen Ther 19:107-112 (2022) doi: 10.1016/j.reth.2022.01.004
https://www.sciencedirect.com/science/a ... 0422000049
He B, Dong S, Chen Q, Dong Z, Chen C - "DNA Methylation Profiles of Ovarian Granular Cells from Fluorosis Female Patients Suffering Reproductive Dysfunctions" Biol Trace Elem Res 200(8):3529-3536 (2022) doi: 10.1007/s12011-021-02954-1. Epub 2021 Oct 22
https://pubmed.ncbi.nlm.nih.gov/34686994/
"The methylation sequences of KGN cells relating to autophagy were significantly changed by NaF treatment dose-dependently. Based on these results, it is concluded that DNA methylation and autophagy may play a significant role in the pathophysiology of reproductive dysfunction caused by fluorosis."
Ma Y, Yao Y, Zhong N, Angwa LM, Pei J - "The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats" Environ Toxicol Pharmacol 75:103331 (2020) doi: 10.1016/j.etap.2020.103331. Epub 2020 Jan 23
https://www.sciencedirect.com/science/a ... via%3Dihub
"An increase in BMP-2 expression was also observed with an increase of fluoride exposure time...It concludes that fluoride has a dose-response effect on BMP-2 in fluorosis rats."
https://www.sciencedirect.com/science/a ... 8920301873
Pan X, Yan W, Qiu B, Liao Y, Liao Y, Wu S, Ming J, Zhang A - "Aberrant DNA methylation of Cyclind-CDK4-p21 is associated with chronic fluoride poisoning" Chem Biol Interact 315:108875 (2020) doi: 10.1016/j.cbi.2019.108875. Epub 2019 Oct 25
https://www.sciencedirect.com/science/a ... 9719316023
Wang A, Ma Q, Gong B, Sun L, Afrim FK, Sun R, He T, Huang H, Zhu J, Zhou G, Ba Y - "DNA methylation and fluoride exposure in school-age children: Epigenome-wide screening and population-based validation" Ecotoxicol Environ Saf 223:112612 (2021) doi: 10.1016/j.ecoenv.2021.112612. Epub 2021 Aug 6
https://www.sciencedirect.com/science/a ... 1321007247
Organic Fluoride
Silva AS, Toffoli LV, Estrada VB, Veríssimo LF, Francis-Oliveira J, Moreira EG, Gomes MV, Pelosi GG - "Maternal exposure to fluoxetine during gestation and lactation induces long lasting changes in the DNA methylation profile of offspring's brain and affects the social interaction of rat" Brain Res Bull. 142:409-413 (2018) doi: 10.1016/j.brainresbull.2018.09.007. Epub 2018 Sep 17
https://www.sciencedirect.com/science/a ... via%3Dihub
Toffoli LV, Rodrigues GM Jr, Oliveira JF, Silva AS, Moreira EG, Pelosi GG, Gomes MV - "Maternal exposure to fluoxetine during gestation and lactation affects the DNA methylation programming of rat's offspring: modulation by folic acid supplementation" Behav Brain Res 265:142-7 (2014) doi: 10.1016/j.bbr.2014.02.031. Epub 2014 Feb 28
https://www.sciencedirect.com/science/a ... via%3Dihub
https://www.sciencedirect.com/science/a ... 972033120X
"At 144 h, several genes related to thyroid hormones that are essential for neurodevelopment, including corticotropin releasing hormone b (crhb), iodothyronine deiodinase 3a (dio3a), thyroid-stimulating hormone receptor (tshr) and nkx2 homeobox1 (nkx 2.1), were up-regulated by 8:8 PFPiA at 5.79 μM."
==> "An increasing number of epidemiological, mammalian, and alternative fish model studies have reported relationships between PFAS exposure and DNA methylation (Blanc et al., 2019; Bouwmeester et al., 2016; Miura et al., 2018; Tian et al., 2019; Wan et al., 2010; Watkins et al., 2014)."
===
Just a short list of relevant papers:
Bunker SK, Dandapat J, Chainy GBN, Sahoo SK, Nayak PK - "Neonatal Exposure to 6-n-Propyl-Thiouracil, an Anti-Thyroid Drug, Alters Expression of Hepatic DNA Methyltransferases, Methyl CpG-Binding Proteins, Gadd45a, p53, and PCNA in Adult Male Rats" Eur Thyroid J 6(6):281-291 (2017)
doi: 10.1159/000479681
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704726/
"The results suggest that neonatal exposure of male rats to PTU resulted in alteration in the expression of proteins that are associated with DNA methylation and genome stabilization in adult rat liver."
Lafontaine N, Campbell PJ, Castillo-Fernandez JE, Mullin S, Lim EM, Kendrew P, Lewer M, Brown SJ, Huang RC, Melton PE, Mori TA, Beilin LJ, Dudbridge F, Spector TD, Wright MJ, Martin NG, McRae AF, Panicker V, Zhu G, Walsh JP, Bell JT, Wilson SG - "Epigenome-Wide Association Study of Thyroid Function Traits Identifies Novel Associations of fT3 With KLF9 and DOT1L" J Clin Endocrinol Metab 106(5):e2191-e2202 (2021) doi: 10.1210/clinem/dgaa975
https://academic.oup.com/jcem/article/1 ... 91/6114572
"We identified 23 differentially methylated regions associated with fT3, fT4, or TSH...This study has demonstrated associations between blood-based DNAm and both fT3 and TSH."
Qu M, Wan S, Wu H, Ren B, Chen Y, Liu L, Shen H - "The Whole Blood DNA Methylation Patterns of Extrinsic Apoptotic Signaling Pathway Related Genes in Autoimmune Thyroiditis among Areas with Different Iodine Levels" Br J Nutr 9:1-35 (2022). doi: 10.1017/S0007114522000721
https://www.cambridge.org/core/journals ... 53D0CB4D56
Wan S, Liu L, Ren B, Qu M, Wu H, Jiang W, Wang X, Shen H - "DNA Methylation Patterns in the HLA-DPB1 and PDCD1LG2 Gene Regions in Patients with Autoimmune Thyroiditis from Different Water Iodine Areas" Thyroid 31(11):1741-1748 (2021)
https://www.liebertpub.com/doi/10.1089/thy.2021.0221
" There were multiple differential methylation sites in the HLA-DPB1 and PDCD1LG2 genes between the case and control population with different water iodine levels."
- SEE also:
Chen Y, Liu J, Qu M, Ren B, Wu H, Zhang L, Zhou Z, Liu L, Shen H - "DNA Methylation of KLRC1 and KLRC3 in Autoimmune Thyroiditis: Perspective of Different Water Iodine Exposure" Biomed Environ Sci 37(9):1044-1055 (2024)
"The DNA methylation status of KLRC1 and KLRC3 may play crucial roles in AIT pathogenesis. Additionally, KLRC1 methylation is influenced by varying iodine concentrations in water."
https://www.tandfonline.com/doi/abs/10. ... ode=iaut20
"Multiple regression analysis indicated that patients with HD, including severe or mild HD, showed higher methylation levels at the -426 CpG site than control subjects. Patients with intractable GD showed lower methylation levels at the -664 and -666 CpG sites than patients with GD in remission. In conclusion, IL6 gene methylation levels were related to the susceptibility to HD and the intractability of GD."
Morita E, Watanabe M, Inoue N, Hashimoto H, Haga E, Hidaka Y, Iwatani Y - "Methylation levels of the TNFA gene are different between Graves' and Hashimoto's diseases and influenced by the TNFA polymorphism" Autoimmunity 51(3):118-125 (2018)
https://www.tandfonline.com/doi/abs/10. ... 18.1448078
Luo J, Wang X, Yuan L, Guo L - "Genome‑wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism" Clin Epigenetics 13(1):123 (2021) doi: 10.1186/s13148-021-01109-2
https://clinicalepigeneticsjournal.biom ... 21-01109-2
Fluoride
Short list:
Chen N, Zhang J, Yin C, Liao Y, Song L, Hu T, Pan X - "Abnormal methylation of Mill1 gene regulates osteogenic differentiation involved in various phenotypes of skeletal fluorosis in rats and methionine intervention" Ecotoxicol Environ Saf 290:117519 (2024) doi: 10.1016/j.ecoenv.2024.117519.
https://www.sciencedirect.com/science/a ... 1324015951
"The results indicated that under normal nutrition and fluoride exposure followed by methionine intervention, the methylation levels of the Mill1 gene increased, whereas its high expression and enhanced osteogenic differentiation were restrained."
Chen L, Yin N, Ding Y, Zhang ML, Li M, Zhong JJ, Feng SM - "Effects of fluoride on the proliferation and activation of osteoblasts by regulating methylation of the DNA repair genes MGMT and MLH1" Regen Ther 19:107-112 (2022) doi: 10.1016/j.reth.2022.01.004
https://www.sciencedirect.com/science/a ... 0422000049
He B, Dong S, Chen Q, Dong Z, Chen C - "DNA Methylation Profiles of Ovarian Granular Cells from Fluorosis Female Patients Suffering Reproductive Dysfunctions" Biol Trace Elem Res 200(8):3529-3536 (2022) doi: 10.1007/s12011-021-02954-1. Epub 2021 Oct 22
https://pubmed.ncbi.nlm.nih.gov/34686994/
"The methylation sequences of KGN cells relating to autophagy were significantly changed by NaF treatment dose-dependently. Based on these results, it is concluded that DNA methylation and autophagy may play a significant role in the pathophysiology of reproductive dysfunction caused by fluorosis."
Ma Y, Yao Y, Zhong N, Angwa LM, Pei J - "The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats" Environ Toxicol Pharmacol 75:103331 (2020) doi: 10.1016/j.etap.2020.103331. Epub 2020 Jan 23
https://www.sciencedirect.com/science/a ... via%3Dihub
"An increase in BMP-2 expression was also observed with an increase of fluoride exposure time...It concludes that fluoride has a dose-response effect on BMP-2 in fluorosis rats."
- Compare: Deng T, Zhang W, Zhang Y, Zhang M, Huan Z, Yu C, Zhang X, Wang Y, Xu J - "Thyroid-stimulating hormone decreases the risk of osteoporosis by regulating osteoblast proliferation and differentiation" BMC Endocr Disord 21(1):49 (2021) doi: 10.1186/s12902-021-00715-8
"Bone morphogenetic protein (BMP)2 activity was enhanced with both increased TSH concentration and increased time."
https://www.sciencedirect.com/science/a ... 8920301873
Pan X, Yan W, Qiu B, Liao Y, Liao Y, Wu S, Ming J, Zhang A - "Aberrant DNA methylation of Cyclind-CDK4-p21 is associated with chronic fluoride poisoning" Chem Biol Interact 315:108875 (2020) doi: 10.1016/j.cbi.2019.108875. Epub 2019 Oct 25
https://www.sciencedirect.com/science/a ... 9719316023
Wang A, Ma Q, Gong B, Sun L, Afrim FK, Sun R, He T, Huang H, Zhu J, Zhou G, Ba Y - "DNA methylation and fluoride exposure in school-age children: Epigenome-wide screening and population-based validation" Ecotoxicol Environ Saf 223:112612 (2021) doi: 10.1016/j.ecoenv.2021.112612. Epub 2021 Aug 6
https://www.sciencedirect.com/science/a ... 1321007247
Organic Fluoride
Silva AS, Toffoli LV, Estrada VB, Veríssimo LF, Francis-Oliveira J, Moreira EG, Gomes MV, Pelosi GG - "Maternal exposure to fluoxetine during gestation and lactation induces long lasting changes in the DNA methylation profile of offspring's brain and affects the social interaction of rat" Brain Res Bull. 142:409-413 (2018) doi: 10.1016/j.brainresbull.2018.09.007. Epub 2018 Sep 17
https://www.sciencedirect.com/science/a ... via%3Dihub
Toffoli LV, Rodrigues GM Jr, Oliveira JF, Silva AS, Moreira EG, Pelosi GG, Gomes MV - "Maternal exposure to fluoxetine during gestation and lactation affects the DNA methylation programming of rat's offspring: modulation by folic acid supplementation" Behav Brain Res 265:142-7 (2014) doi: 10.1016/j.bbr.2014.02.031. Epub 2014 Feb 28
https://www.sciencedirect.com/science/a ... via%3Dihub
- SEE: Fluoxetine (Prozac), Thyroid & Fluoride
viewtopic.php?f=7&t=5813
(More info; Gq/11)
https://www.sciencedirect.com/science/a ... 972033120X
"At 144 h, several genes related to thyroid hormones that are essential for neurodevelopment, including corticotropin releasing hormone b (crhb), iodothyronine deiodinase 3a (dio3a), thyroid-stimulating hormone receptor (tshr) and nkx2 homeobox1 (nkx 2.1), were up-regulated by 8:8 PFPiA at 5.79 μM."
==> "An increasing number of epidemiological, mammalian, and alternative fish model studies have reported relationships between PFAS exposure and DNA methylation (Blanc et al., 2019; Bouwmeester et al., 2016; Miura et al., 2018; Tian et al., 2019; Wan et al., 2010; Watkins et al., 2014)."
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