https://doi.org/10.13701/j.cnki.kqyxyj.2025.05.011
Abstract:
Objective: To analyze the effects of sodium fluoride (NaF) on gene expression in mouse ameloblast LS8 cells using transcriptome sequencing (RNA-seq), and to identify key differentially expressed genes (DEGs) that may reveal mechanisms underlying dental fluorosis.
Methods: LS8 cells were divided into control and NaF groups (1.5 mmol/L, 24 h). RNA-seq was performed to identify DEGs, followed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. RT-qPCR validated gene expression results, and Western blotting assessed pathway protein expression.
Results: A total of 104 DEGs were detected, including 34 upregulated and 70 downregulated genes. KEGG analysis showed significant enrichment of PI3K/Akt and MAPK signaling pathways. RT-qPCR confirmed decreased mRNA levels of PI3K, Akt, and MAPK (P < 0.05), consistent with RNA-seq data. Western blotting revealed reduced relative expression of p-PI3K/PI3K, p-Akt/Akt, and p-p44/p42 MAPK/p44/p42 MAPK (P < 0.05).
Conclusion: PI3K/Akt and MAPK signaling pathways may be involved in regulating the cytotoxic effects of fluoride on ameloblasts.