2011 - fluoride effects on the thyroid

[admin]

李建珍;;高氟对人甲状腺细胞功能的影响[J];武警医学;2011年01期

高氟对人甲状腺细胞功能的影响
李建珍
【摘要】：目的探讨高氟对人甲状腺细胞功能的影响。方法用人甲状腺细胞,采用原代培养方法,按氟(NaF)剂量不同分为:0(对照组)、40、80、160 mg/L组。染氟48 h后,采用噻唑蓝(MTT)法测定细胞存活率,化学发光法测定抗甲状腺过氧化物酶抗体(TPOA)水平和荧光免疫分析法测定血清总甲状腺激素(T_4)水平。结果甲状腺细胞染氟培养48 h后,细胞存活率组间比较差异有统计学意义(P0.05);40 mg/L组细胞存活率与对照组(100)%比较,差异无统计学意义(P0.05);80、160 mg/L组细胞存活率分别与对照组比较,差异有统计学意义(P0.05)。甲状腺细胞T_4水平随着染氟剂量的升高而明显降低,组间比较差异有统计学意义(P0.05)。甲状腺细胞TPOA表达水平也随着染氟剂量的升高而明显增加,组间比较差异有统计学意义(P0.05)。结论高氟可损伤甲状腺细胞,降低细胞存活率,提高TTPOA表达,降低T_4水平。
【作者单位】： 武警山西总队医院检验科;
【关键词】： 氟 甲状腺 甲状腺激素 甲状腺过氧化物酶抗体
【分类号】：R581
【正文快照】：
地方性氟中毒与甲状腺肿的流行有很大的重叠性，氟化物可导致动物甲状腺肿大，长期高氟摄人可引起甲状腺的形态结构和甲状腺机能的严重损害，对甲状腺功能正常发挥影响较大「‘一，」。为此，笔者采用细胞培养方法，观察高剂量氟(氟化钠，NaF)对原代培养人甲状腺细胞存活率、
http://www.cnki.com.cn/Article/CJFDTOTA ... 101019.htm

"Armed Medicine" 2011 01 Add to Favorite Get Latest Update
Fluoride on human thyroid cell function
Li Jianzhen
Abstract: Objective To investigate the fluoride on human thyroid cell function. Methods employing thyroid cells, using primary culture method, according fluoride (NaF) is divided into different doses: 0 (control group), 40,80,160 mg / L group. After 48 h exposed to fluoride, using MTT (MTT) cell viability was determined, chemiluminescence anti-thyroid peroxidase antibodies (TPOA) levels and fluorescence immunoassay method for measuring serum total thyroid hormone (T_4) levels. Results thyroid cells after 48 h culture exposed to fluoride, cell survival difference between groups was statistically significant (P0.05); 40 mg / L group, cell viability and the control group (100)%, the difference was not statistically significant ( P0.05); 80,160 mg / L of cell survival rates were compared with the control group, the difference was statistically significant (P0.05). T_4 levels of thyroid cells transfected with elevated fluoride doses significantly reduced, the difference between groups was statistically significant (P0.05). TPOA thyroid cells transfected with expression levels were also elevated fluoride doses significantly increased, the difference between groups was statistically significant (P0.05). Conclusion fluoride can damage the thyroid cells, reducing cell viability, improve TTPOA expression T_4 lower level.
【Key Words】 : People's Armed Police Corps Hospital of Shanxi Province;
Key words : Fluoride thyroid thyroid hormone thyroid peroxidase antibody
【CateGory Index】: R581 【Key Words】:
Endemic goitre prevalence of fluorosis and have great overlap, fluoride can cause goiter animals, long-term fluoride intake can cause thyroid morphology and thyroid function badly damaged to make an impact on thyroid function was normal compared with Big "'a,." To this end, the author uses cell culture method, the effect of high doses of fluoride (sodium fluoride, NaF) on primary cultured human thyroid cell viability,