2016 - Oxidative Stess and F- induced thyroid injury

2016 - Oxidative Stess and F- induced thyroid injury

Postby admin » Fri Jul 17, 2020 12:32 am

崔玉山,赵亮,曾强,等.氧化应激在高碘高氟致甲状腺损伤中的作用[J].环境与职业医学,2016(1):7-12
http://www.cqvip.com/QK/98530A/201601/667712011.html

Cui Yushan, Zhao Liang, Zeng Qiang, Wang Rui, Zhang Lei, Fu Gang, Feng Baojia, Liu Hongliang - "The role of oxidative stress in iodine and fluoride induced thyroid injury" "Environmental and Occupational Medicine (1):7-12 (2016)
http://www.cqvip.com/QK/98530A/201601/667712011.html

论文服务:
摘 要:[目的]探讨氧化应激在高碘和(或)高氟致甲状腺损伤中的作用。[方法]建立50 mmol/L KI(高碘)、1 mmol/L NaF(高氟)、50 mmol/L KI+1 mmol/L NaF(高碘高氟)暴露的甲状腺细胞模型和含1.685 mg/L KIO3自来水(高碘)、含20 mg/L NaF自来水(高氟)、含1.685 mg/L KIO3+20 mg/L NaF自来水(高碘高氟)暴露的Wistar大鼠模型并开展人群流行病学调查,采用噻唑蓝(MTT)法、流式细胞术、分光光度法、电极法、比色法、放射法等检测细胞活性、活性氧(ROS)水平,大鼠尿碘、尿氟、血液丙二醛(MDA)、超氧化物歧化酶(SOD)水平,人血清ROS及三碘甲状腺素(T3)、甲状腺素(T4)和促甲状腺激素(TSH)水平。[结果]对照组细胞的活力为(100.00±0.00)%,而高碘、高氟及高碘高氟组下降为(73.54±8.37)%、(84.54±7.55)%和(72.62±7.15)%(P〈0.05),且高碘和(或)高氟组均可改变细胞形态;相对于对照组细胞的ROS荧光度(1 409.50±208.46),高氟及高碘高氟组的ROS荧光度上升,分别为2 304.15±390.47和2 669.24±646.80(P〈0.05)。高碘及高碘高氟联合均明显升高大鼠尿碘水平(P〈0.05);高氟及高碘高氟联合明显升高大鼠尿氟水平(P〈0.05);高碘和(或)高氟可改变大鼠甲状腺滤泡形状;雄性对照组大鼠MDA水平为(3.00±0.33)μmol/L,高氟组大鼠MDA水平上升为(4.27±0.82)μmol/L(P〈0.05),同时雄性对照组大鼠SOD水平为(300.92±36.02)×10^3 U/L,而高氟组下降为(226.33±41.21)×10^3 U/L(P〈0.05)。儿童高碘高氟组T4浓度明显上升(P〈0.05);高氟及高碘高氟组儿童血清ROS水平由对照组的(72.83±13.70)×10^3 IU/L上升为(76.65±125.45)×10^3 IU/L及(89.95±63.85)×10^3 IU/L(P〈0.05)。[结论]高碘和(或)高氟可造成甲状腺损伤,未发现高碘可致机体氧化应激,但氧化应激参与了高氟及高碘高氟致甲状腺损伤的过程。

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Re: 2016 - Oxidative Stess and F- induced thyroid injury

Postby admin » Fri Jul 17, 2020 12:33 am

Summary:[Objective] To explore the role of oxidative stress in thyroid injury caused by high iodine and/or high fluoride. [Method] Establish a model of thyroid cells exposed to 50 mmol/L KI (high iodine), 1 mmol/L NaF (high fluoride), 50 mmol/L KI + 1 mmol/L NaF (high iodine and high fluoride) and contain 1.685 mg/L KIO3 tap water (high iodine), Wistar rat model with 20 mg/L NaF tap water (high fluoride) and 1.685 mg/L KIO3+20 mg/L NaF tap water (high iodine and high fluoride) exposure and conduct population epidemiological investigation, Cellular activity, reactive oxygen species (ROS) levels were detected by thiazole blue (MTT) method, flow cytometry, spectrophotometry, electrode method, colorimetry, radiometry, etc. Rat urine iodine, urine fluoride, blood malondialdehyde (MDA), superoxide dismutase (SOD) levels, human serum ROS and triiodothyroxine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) levels. [Result] The viability of the cells in the control group was (100.00±0.00)%, while the decrease in the high iodine, high fluoride and high iodine high fluoride group was (73.54±8.37)%, (84.54±7.55)% and (72.62±7.15)% (P<0.05), and the high iodine and/or high fluoride group can change the cell morphology; the ROS fluorescence of the control group cells (1 409.50±208.46), the high fluoride and high iodine high fluoride group ROS fluorescence The increase was 2 304.15±390.47 and 2 669.24±646.80 (P<0.05). The combination of high iodine and high iodine and high fluoride significantly increased the urinary iodine level of rats (P<0.05); the combination of high fluoride and high iodine and high fluoride significantly increased the urinary fluoride level of rats (P<0.05); high iodine and (or) high Fluorine can change the shape of thyroid follicles in rats; the MDA level of male control rats is (3.00±0.33) μmol/L, and the MDA level of rats in the high fluoride group rises to (4.27±0.82) μmol/L (P<0.05), At the same time, the SOD level of male control rats was (300.92±36.02)×10^3 U/L, while the high fluoride group decreased to (226.33±41.21)×10^3 U/L (P<0.05). The concentration of T4 in children with high iodine and high fluoride group increased significantly (P<0.05); the serum ROS level in children with high fluoride and high iodine and high fluoride group increased from (72.83±13.70)×10^3 IU/L of the control group to (76. 65±125.45)×10^3 IU/L and (89.95±63.85)×10^3 IU/L (P<0.05). [Conclusion] High iodine and/or high fluoride can cause thyroid damage. High iodine has not been found to cause oxidative stress in the body, but oxidative stress is involved in the process of thyroid damage caused by high fluoride and high iodine and high fluoride.
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