2009 - FX of fluoride on TH gene expression - FRTL cells

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118. 江鹏;张维东;柴春彦;肖睿;丁明星;刘国艳;;氟化物对FRTL细胞甲状腺激素代谢相关基因表达的影响[J];中国兽医学报;2009年07期
http://www.cnki.com.cn/Article/CJFDTOTA ... 907018.htm
Jiang Peng et al. - "Effects of fluoride on thyroid hormone metabolism in FRTL cells and related gene expression" Chinese Journal of Veterinary Science (07)(2009)

为探讨氟对FRTL细胞甲状腺球蛋白(TG),甲状腺过氧化物酶(TPO)和钠碘转运体(NIS)基因表达的影响,传代培养FRTL细胞,在对数生长期用氟化钠处理,使培养液中的氟浓度分别为20、10、5、2.5、1.25 mg/L,同时设空白对照细胞。培养72 h后收集细胞;采用半定量RT-PCR分析FRTL细胞TG、TPO、NIS mRNA和-βactin表达水平。试验结果表明,与对照细胞相比,较低浓度氟化钠处理的FRTL细胞TG基因表达量代偿性升高,随氟质量浓度增加(5.0 mg/L以上),TG表达量显著降低(P0.05);各个浓度氟化钠处理的FRTL细胞TPO、NIS基因表达量均下降或显著下降(P0.05)。由此可知,氟化物降低了甲状腺细胞TG、TPO、NIS基因表达水平,造成甲状腺摄取和利用碘、甲状腺激素合成、贮存、分泌功能障碍,进而导致甲状腺结构和功能异常。

Abstract：To investigate the effects of fluoride on the expression of thyroglobulin (TG)，thyroid peroxidase
(TPO)，odium iodide symporter(NIS)genes in FRTL cells，FRTL cells cultured in vitro were treated in logarithmic
phase with sodium fluoride at different concentration of 2O．0，l0．0，5．0，2．5，1．25 mg／L，respectively．After 72 h，
the cells were collected and semi—quantitative RT-PCR for TG mRNA，TPO mRNA and NIS ITIRNA and 8一aetin、=|，as
performed．The results showed that compared with the control cells，the expression levels of TG gene in FRTL ceils
treated with lower concentration of sodium fluoride increased compensatively，but decreased significantly(P<O．05)
with higher concentration(> 5．0 mg／L)：the RT～PCR products of TPo and NIS in FRTL cells treated with all con—
centration of sodium fluoride reduced，some of them were significant(P< O．O5)．It iS concluded that fluoride can re—
duce the expression of TG ，TPO ，NIS genes in thyroid cells，consequently cause dysfunction of thyroid in uptaking
and unilizing of iodine，and synthesizing，storing，secreting of thyroid hormones．