60. 江鹏 , 丁明星, 刘国艳, 张维东, 宋巍巍, 柴春彦, 氟化钠对原代培养大鼠甲状腺细胞形态学的影响, 中国地方病学杂志 2008年27卷05期 484-487页
http://new.med.wanfangdata.com.cn/Paper ... z200805004
Jiang et al. - ""Effects of sodium fluoride on the morphologic characteristics of primarily cultured thyroid cells of rats" Chinese Journal of Endemiology 27(5)(2008)
目的 观察不同染氟(氟化钠)剂量对体外培养原代SD大鼠甲状腺细胞形态学的影响,为探讨氟引起甲状腺组织损伤提供理论依据.方法 采用原代细胞培养方法,体外培养SD大鼠甲状腺细胞,96 h后收集对数生长期的细胞,将细胞密度调整约5.0×108个/L;取5 ml(约 2.5×106个细胞)加入到6孔培养板中,培养12h,按不同染氟剂量分为0(对照)、10、100、1000 μmol/L组,每组设6个重复样.相差显微镜下观察甲状腺细胞的形态变化.48 h后收集细胞,扫描电镜下进行甲状腺形态学观察和分析.结果 相差显微镜下,对照组甲状腺细胞透明度良好.聚集成群,贴壁良好,细胞存活率在90%以上;染氟组甲状腺细胞有大量悬浮,透明度差,细胞存活率约55%.扫描电镜下,可见对照组甲状腺细胞胞膜完整,细胞之间嵌合紧密,界限清晰,分裂良好;10、100μmol/L组甲状腺细胞明显皱缩和变形;1000μmo/L组甲状腺细胞胞膜不完整,集结在一起生长的细胞间界限不明显,有的细胞发生明显皱缩,有的细胞则完全破碎.结论 氟影响甲状腺细胞的生长和发育,使细胞的形态发生改变,损伤程度与剂量有关.
"Effects of sodium fluoride on the morphologic characteristics of primarily cultured thyroid cells of rats" CHINESE JOURNAL OF ENDEMIOLOGY
Issue: Volume 27, Issue 05, 2008
http://eng.med.wanfangdata.com.cn/Paper ... z200805004
Authors: JIANG Peng(Department of Surgery,School of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)
DING Ming-xing(Department of Surgery,School of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)
SONG Wei-wei(Department of Surgery,School of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)
Abstract： Objective To investigate the effects of different concentrations of sodium fluoride on the morphologic characteristics of primarily cultured thyroid cells of SD rats and in order to obtain important proof for approaehing the mechani8m of thyroid gland damage caused by fluoride.Methods Thyroid cells of SD rat were primarily culture for 96 hours,and cell density was adjusted to 5.0×108/L Cell suspension with 5 ml Wills seeded into 6 weII plates,after 12 hours,0(contr01),10.100,1000 μmol/L of sodium fluoride was added into the well, witll each well representing different level of treatment group.Finally the cultured thyroid cells were collected for morph010gic study.Results Under microscope,the transparency of the control thyroid cells Was good,and cells gathered in cluster and adhered to wall.But a lot of cells treated with fluoride suspended,and lost their transparency-under scaning delectron microscope,the control calls showed integrated membrane and tightness to each other,as well as clear boundary between cells normal proliferation.While the thyroid cells treated with 10,100 μmol/L sodium fluoride 0bviouslv shrinked and deformed,and the cells treated with 1000 μmol/L of sodium fluoride were broken-Conclusions fluoride can affect the growth and development of thyroid cell and damage the structure and morphology.Sodium fluoride affects the morphologic characteristics of thyroid cells in a dose-response manner.