http://www.cnki.com.cn/Article/CJFDTOTA ... 101019.htm
Li J - "Effects of fluoride on human thyroid cell function"Medical Journal of the Chinese People's Armed Police Forces (01) (2011)
摘要 目的 探讨高氟对人甲状腺细胞功能的影响.方法 用人甲状腺细胞,采用原代培养方法,按氟(NaF)剂量不同分为:0(对照组)、40、80、160 mg/L组.染氟48 h后,采用噻唑蓝(MTT)法测定细胞存活率,化学发光法测定抗甲状腺过氧化物酶抗体(TPOA)水平和荧光免疫分析法测定血清总甲状腺激素(T4)水平.结果 甲状腺细胞染氟培养48 h后,细胞存活率组间比较差异有统计学意义(P＜0.05);40 mg/L组细胞存活率与对照组(100)%比较,差异无统计学意义(P＞0.05);80、160 mg/L组细胞存活率分别与对照组比较,差异有统计学意义(P＜0.05).甲状腺细胞T4水平随着染氟剂量的升高而明显降低,组间比较差异有统计学意义(P＜0.05).甲状腺细胞TPOA表达水平也随着染氟剂量的升高而明显增加,组间比较差异有统计学意义(P＜0.05).结论 高氟可损伤甲状腺细胞,降低细胞存活率,提高TPOA表达,降低T4水平.
Objective To investigate the effects of fluoride on human thyroid cell function method employing thyroid cells, using primary culture method, according to fluoride (NaF) divided into doses: 0 (control group), 40,80,160 mg / L group. After dyeing fluorine 48 h, using the MTT (MTT) measured antithyroid peroxidase antibodies (TPOA) cell viability, chemiluminescence serum total thyroid hormone (T4) levels and the level of fluorescence immunoassay assay results of thyroid Cells cultured 48 h after transfection fluoride, cell survival between groups was statistically significant difference (P <0.05); 40 mg / L of cell survival and control group (100)%, the difference was not statistically significant (P> 0.05); 80,160 mg / L, respectively, comparing cell survival group and the control group, the difference was statistically significant (P <0.05) levels of thyroid T4 cells transfected with increasing doses of fluoride significantly reduced, the difference between groups. There was statistically significant (P <0.05). TPOA expression levels of thyroid cells transfected with increasing doses of fluoride significantly increased, the difference between groups was statistically significant (P <0.05). Conclusions fluoride can damage the thyroid cells, reduce cell viability, improve TPOA expression, reduced T4 levels.