163. 氟对大鼠甲状腺形态和甲状腺过氧化物酶活性及蛋白表达的影响 中国地方病学杂志2012年31卷03期 271-274页
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Li Hong, Cai Qi, Wang D - "Effects of fluoride on rat thyroid morphology, thyroid peroxidase activity and the expression of thyroid peroxidase protein" Chinese Journal of Medicine 31(3):271-274 (2012)
目的 观察长期氟过量对大鼠甲状腺形态结构、甲状腺过氧化物酶(TPO)活性及TPO蛋白表达的影响,并探讨其可能的作用机制.方法 选用雄性SD大鼠40只,按体质量随机分为对照组、低氟组、中氟组、高氟组,每组10只.4组大鼠分别饮用含氟0.40(普通自来水)、15.00、30.00、60.00 mg/L的自来水,均食用普通粮食配制的饲料.喂养180 d后,将大鼠麻醉,取甲状腺组织.在400倍光镜下观察甲状腺组织形态学改变;改良愈创木酚法测定TPO活性；免疫印迹(Western blot)法检测TPO蛋白质表达水平.结果 光镜下,对照组甲状腺滤泡上皮细胞多为单层柱状或立方状,滤泡腔内充满粉红色胶质;低氟组甲状腺滤泡上皮细胞增生活跃;中氟组滤泡增大,滤泡腔内充满深染、黏稠的胶质滤泡；高氟组滤泡上皮细胞明显扁平,滤泡胶质过度浓集,少量滤泡腔甚至有融合,形成巨形滤泡或囊腔.对照组、低氟组、中氟组和高氟组甲状腺细胞TPO活性[(1.572±0.046)、(1.414±0.086)、(1.322±0.049)、(0.960±0.083)U/L]依次降低,组间两两比较差异有统计学意义(P均＜ 0.05)；TPO蛋白表达水平(0.335±0.011、0.156±0.027、0.084±0.020、0.045±0.002)依次下降,组间两两比较差异有统计学意义(P均＜ 0.05).结论 长期摄入过量氟可抑制甲状腺TPO活性和TPO蛋白的表达,进而影响甲状腺组织学改变.
ABSTRACT: Objective To observe the effect of long-term fluoride excess on rat thyroid morphology, thyroid peroxidase (TPO) activity and the expression of TPO protein, and to explore its possible mechanism of action.Methods Forty male SD rats were randomly divided into four groups by weight: control group, low-fluoride group, moderate-fluoride group and high-fluoride group (n = 10), and they were fed with ordinary tap water containing fluorine 0.40,15.00,30.00,60.00 mg / L, respectively, and ate ordinary food prepared feed.After fed for 180 days, rats were anesthetized, and thyroid was taken.The morphology of thyroid was observed under light microscope.TPO activity was measured with improved guaiacol method.The expression of TPO protein was evaluated by Western blotting. Results The thyroid histopathology results show: in control group, the thyroid follicular epithelial cells were columnar or cuboidal, with the follicular cavity filled with pink gum; in low-fluoride group, the thyroid follicular epithelial cell presented active hyperplasia; in moderate-fluoride group , the size of follicular increased, and follicular cavity was filled with dark, sticky colloid follicular; follicular increases, follicular cavity filled with dark, sticky colloid follicular; in high-fluoride group, the follicular epithelial cells showed apparent flat shape and excessive concentration of follicular colloid, a small amount of follicular lumen even showed the phenomenon of fusion, forming a giant follicular or cystic cavity.Among the four groups of control group, low-fluoride group, moderate-fluoride group and high-fluoride group, with increased fluoride , TPO activity [(1.572 ± 0.046), (1.414 ± 0.086), (1.322 ± 0.049), (0.960 ± 0.083) U / L] decreased, and the differences were statistically significant between the two groups (all P <0.05). With increased fluoride, the expression of TPO protein (0.335 ± 0.011,0.156 ± 0.027,0.084 ± 0.020,0.045 ± 0.002) decreased, and the differences were statistically significant between the two groups (all P <0.05). Conclusioons Long-term intake of excessive fluoride can inhibit the thyroid TPO activity and the expressions of TPO as well as thyroid hormone synthesis, and leads to histological changes in rat thyroid.