Yang H, Xing R, Liu S, Yu H, Li P - "γ-Aminobutyric Acid Ameliorates Fluoride-Induced Hypothyroidism in Male Kunming Mice" Life Sci. 2015 Dec 24. pii:
S0024-3205(15)30135-1. doi: 10.1016/j.lfs.2015.12.041. [Epub ahead of print]
PubMed PMID: 26724496.
AIM: This study evaluated the protective effects of γ-aminobutyric acid (GABA), a
non-protein amino acid and anti-oxidant, against fluoride-induced hypothyroidism
MAIN METHODS: light microscope sample preparation technique and TEM sample
preparation technique were used to assay thyroid microstructure and
ultrastructure; enzyme immunoassay method was used to assay hormone and protein
levels; immunohistochemical staining method was used to assay apoptosis of
thyroid follicular epithelium cells.
KEY FINDINGS: Subacute injection of sodium fluoride (NaF) decreased blood T4, T3
and thyroid hormone-binding globulin (TBG) levels to 33.98 μg/L, 3 2.8 ng/ml and
11.67 ng/ml, respectively. In addition, fluoride intoxication induced structural
abnormalities in thyroid follicles. Our results showed that treatment of
fluoride-exposed mice with GABA appreciably decreased metabolic toxicity induced
by fluoride and restored the microstructural and ultrastructural organisation of
the thyroid gland towards normalcy. Compared with the negative control group,
GABA treatment groups showed significantly upregulated T4, T3 and TBG levels
(42.34 μg/L, 6.54 ng/ml and 18.78 ng/ml, respectively; P < 0.05), properly
increased TSH level and apoptosis inhibition in thyroid follicular epithelial
SIGNIFICANCE: To the best of our knowledge, this is the first study to establish
the therapeutic efficacy of GABA as a natural antioxidant in inducing
thyroprotection against fluoride-induced toxicity.