190. 作者：黄辉 贾新梅 李世 阎平平 程学敏 崔留欣 巴月 COL1A2基因多态性与儿童氟斑牙关系 CHINESE JOURNAL OF PUBLIC HEALTH 年，卷(期)：2007, 23(4)
Huang, Jiaxin Mei, Li Shihong, Yanping Ping, Chengxue Min, Cui Liuxin - "COL1A2 gene polymorphism and the relationship to dental fluorosis" Chinese J Public Health 23(4) (2007)
目的 调查Ⅰ型胶原α2链(COL1A2)PvuⅡ基因型在饮水氟、碘浓度不同地区儿童中的分布,探讨COL1A2 PvuⅡ基因多态性与儿童氟斑牙的关系.方法 采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测氟碘正常区、单纯高氟区和高氟高碘区8～12岁儿童的COL1A2 PvuⅡ基因型.Dean氏法检查儿童氟斑牙患病情况.结果 3区儿童COL1A2 PvuⅡ基因型在氟碘正常地区分布频率分别为PP23.3%,Pp36.7%,pp40.0%;单纯高氟区分布频率分别为PP9.8%,Pp45.1%,pp45.1%,高氟高碘区分布频率分别为PP13.7%,Pp38.4%,pp47.9%,3区分布差异无统计学意义(χ2=5.191,P＞0.05).儿童氟斑牙患病率分别为0,52.7%,50.7%,差异有统计学意义(χ2=49.081,P＜0.05);单纯高氟区和高氟高碘区氟斑牙患病率差异无统计学意义(χ2=0.060,P＞0.05),但高氟高碘区的氟斑牙指数低于单纯高氟区.结论 COL1A2 PvuⅡ基因多态性与所调查儿童氟斑牙无明显关系.
Objective To investigate Ⅰ collagen α2 chain (COL1A2) PvuⅡ genotypes fluoride in drinking water, iodine concentration of children in different parts of the distribution, COL1A2 PvuⅡ explore the relationship between gene polymorphism and dental fluorosis method using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method for detecting difluoroiodoethylene normal area, COL1A2 PvuⅡ genotype .Dean's method simply checking children in high fluoride areas and high fluoride iodine area for children aged 8 to 12 the prevalence of dental fluorosis. Results area children COL1A2 PvuⅡ genotype frequency in difluoroiodoethylene normal area of PP23.3% respectively, Pp36.7%, pp40.0%; simple frequency distribution of high fluoride areas PP9.8% respectively, Pp45.1%, pp45.1%, the frequency distribution of high fluoride iodine area PP13.7% respectively, Pp38.4%, pp47.9%, 3 zone distribution difference was not statistically significant (χ2 = 5.191, P> 0.05). Children fluorosed Dental prevalence rates were 0,52.7%, 50.7%, and the difference was statistically significant (χ2 = 49.081, P <0.05); simple high fluoride areas and high fluoride iodine area prevalence of dental fluorosis was no significant difference (χ2 = 0.060, P> 0.05), but the index of dental fluorosis Fluoride iodine area is less than pure high fluoride areas. No significant relationship between dental Conclusions COL1A2 PvuⅡ gene polymorphism and the children surveyed fluorine.