http://www.cnki.com.cn/Article/CJFDTOTA ... 406013.htm
Liu G, Jiang P, Chai CY - "Effects of fluoride on the metabolism of thyroid. Function in FRTL" Journal of Shanghai Jiaotong University (Agricultural Science) 32(6) (2014)
氟化物对FRTL细胞甲状腺功能因子代谢的影响
刘国艳 江鹏 柴春彦
【摘要】：为了探讨氟化物对甲状腺功能因子代谢的影响,本实验传代培养FRTL细胞,用氟化钠处理对数生长期的细胞,使培养液中氟水平分别为0、1.25、2.5、5、10、20mg/L。分别于24和72h后收集细胞上清液,测定上清液中游离甲状腺激素FT3和FT4,甲状腺球蛋白(TG)以及甲状腺过氧化物酶(TPO)的活性。实验结果表明,NaF处理FRTL后,细胞上清液中FT3、FT4水平和TPO活性也不同程度降低,且与添加的氟化物浓度呈现显著的负相关(P0.05);上清液中TG的含量除了NaF为2.5mg/L处理组比对照组有所升高外,其他浓度作用的FRLT上清液中TG含量均降低,整体上TG含量与氟化物浓度之间也呈现显著的负相关(P0.05)。氟化物导致FRTL分泌TG不足,TPO活性及FT3、FT4水平下降,从而导致甲状腺功能降低。本实验揭示了氟化物对甲状腺细胞功能因子代谢的影响规律,在一定程度上也为揭示氟致甲状腺肿的机理奠定了基础。
【作者单位】： 上海交通大学农业与生物学院上海市兽医生物技术重点实验室;
Abstract: To investigate the effects of fluoride on the metabolism of thyroid functional factors, this experiment subcultured and cultured FRTL cells. Sodium fluoride was used to treat cells in the logarithmic growth phase, producing fluoride levels in the culture medium of 0, 1.25, 2.5, 5, 10, and 20 mg/L. Cell culture supernatants were collected at 24 h and 72 h, and free thyroid hormones FT3 and FT4, thyroglobulin (TG), and thyroid peroxidase (TPO) activity in the supernatants were measured. The results showed that after NaF treatment of FRTL cells, the FT3 and FT4 levels and TPO activity in the cell supernatant decreased to varying degrees, and showed a significant negative correlation with the added fluoride concentration (P > 0.05). The TG content in the supernatant increased in the 2.5 mg/L NaF treatment group compared with the control group, but at the other concentrations the TG content in the FRTL supernatant decreased; overall, TG content also showed a significant negative correlation with fluoride concentration (P > 0.05). Fluoride causes insufficient TG secretion by FRTL cells and reduces TPO activity and FT3 and FT4 levels, thereby leading to reduced thyroid function. This experiment reveals the pattern by which fluoride affects the metabolism of functional factors in thyroid cells and, to a certain extent, also lays a foundation for elucidating the mechanism of fluoride-induced goiter.
Author affiliations: Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University.