2014 - Fluoride effects on FRTL

2014 - Fluoride effects on FRTL

Postby admin » Tue Jan 05, 2016 3:06 am

183. 氟化物对FRTL细胞甲状腺功能因子代谢的影响 《上海交通大学学报(农业科学版)》 2014年 第06期下载次数
http://www.cnki.com.cn/Article/CJFDTOTA ... 406013.htm
Liu G, Jiang P, Chai CY - "Effects of fluoride on the metabolism of thyroid. Function in FRTL" Journal of Shanghai Jiaotong University (Agricultural Science) 32(6) (2014)

氟化物对FRTL细胞甲状腺功能因子代谢的影响
刘国艳 江鹏 柴春彦
【摘要】:为了探讨氟化物对甲状腺功能因子代谢的影响,本实验传代培养FRTL细胞,用氟化钠处理对数生长期的细胞,使培养液中氟水平分别为0、1.25、2.5、5、10、20mg/L。分别于24和72h后收集细胞上清液,测定上清液中游离甲状腺激素FT3和FT4,甲状腺球蛋白(TG)以及甲状腺过氧化物酶(TPO)的活性。实验结果表明,NaF处理FRTL后,细胞上清液中FT3、FT4水平和TPO活性也不同程度降低,且与添加的氟化物浓度呈现显著的负相关(P0.05);上清液中TG的含量除了NaF为2.5mg/L处理组比对照组有所升高外,其他浓度作用的FRLT上清液中TG含量均降低,整体上TG含量与氟化物浓度之间也呈现显著的负相关(P0.05)。氟化物导致FRTL分泌TG不足,TPO活性及FT3、FT4水平下降,从而导致甲状腺功能降低。本实验揭示了氟化物对甲状腺细胞功能因子代谢的影响规律,在一定程度上也为揭示氟致甲状腺肿的机理奠定了基础。
【作者单位】: 上海交通大学农业与生物学院上海市兽医生物技术重点实验室;

Abstract: To explore the effect of fluoride on thyroid function factor metabolism, this experiment FRTL subculture cells treated with sodium fluoride logarithmic growth phase cells, the culture medium fluorine levels were 0,1.25,2.5,5 , 10,20mg / L. Respectively 24 and cell supernatants were collected after 72h, the supernatant was measured in free thyroid hormone FT3 and FT4, thyroglobulin (TG) and thyroid peroxidase (TPO) activity. Experimental results show that, NaF treated FRTL, cell supernatant FT3, FT4 and TPO activity levels also reduced to varying degrees, and showed a significant negative correlation (P0.05) and added fluoride concentration; supernatant of TG In addition to NaF content of 2.5mg / L treatment groups than in the control group was increased outside, FRLT supernatant TG content of the other effects of the concentration were lower, also showed a significant negative correlation between the whole TG content and fluoride concentration ( P0.05). FRTL fluoride cause inadequate secretion of TG, decreased TPO activity and FT3, FT4 levels, leading to reduced thyroid function. This experiment revealed the influence of fluoride on thyroid function factor cell metabolism, to a certain extent, also to reveal the mechanism of fluorine cause goiter foundation.
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